| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hughes, Jackson C (12)
|
NCWOW |
31.24 |
31.53 |
0.29 |
| 2 |
Cole, Tyrus D (12)
|
NCECA |
32.87 |
33.80 |
0.93 |
| 3 |
Zapler, Ezra N (11)
|
NCECA |
35.42 |
33.86 |
-1.56 |
| 4 |
Reiniche, Ryan J (11)
|
NCWOW |
34.95 |
34.94 |
-0.01 |
| 5 |
Marlow, Tiago V (12)
|
NCECA |
34.74 |
34.99 |
0.25 |
| 6 |
Tillery, Wade H (12)
|
NCWOW |
35.62 |
35.23 |
-0.39 |
| 7 |
House, William E (11)
|
NCUN |
|
36.08 |
- |
| 8 |
Park, Dylan T (11)
|
NCWOW |
41.74 |
38.50 |
-3.24 |
| 9 |
Cook, Tyson G (12)
|
NCECA |
39.21 |
39.05 |
-0.16 |
| 10 |
Mejouev, Maxim (12)
|
NCWOW |
41.12 |
39.23 |
-1.89 |
| 11 |
Pesko, Gavin E (11)
|
NCECA |
39.02 |
39.61 |
0.59 |
| 12 |
Blaylock, Dakota M (11)
|
NCECA |
51.87 |
52.51 |
0.64 |
|
Hawthorne, Maxwell S (11)
|
NCWOW |
33.57 |
DQ |
- |
|
Squires, Arthur James M (12)
|
NCECA |
46.54 |
NS |
- |