| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Arredondo, Nathan B (12)
|
NCWOW |
26.08 |
25.54 |
-0.54 |
| 2 |
Marsteiner, Samuel T (11)
|
NCWAVE |
26.70 |
25.76 |
-0.94 |
| 3 |
Davidson, Aaron M (12)
|
NCWAVE |
27.43 |
26.94 |
-0.49 |
| 4 |
Hawthorne, Maxwell S (12)
|
NCWOW |
26.47 |
27.13 |
0.66 |
| 5 |
Ball, Charles H (11)
|
NCWAVE |
28.54 |
27.83 |
-0.71 |
| 6 |
Handschumacher, Landon D (12)
|
NCWAVE |
30.67 |
28.43 |
-2.24 |
| 7 |
House, William E (12)
|
NCWOW |
28.36 |
28.50 |
0.14 |
| 8 |
Moujeahid, Adam K (12)
|
NCWAVE |
30.71 |
28.85 |
-1.86 |
| 9 |
Reiniche, Ryan J (12)
|
NCWOW |
28.38 |
29.07 |
0.69 |
| 10 |
Park, Dylan T (12)
|
NCWOW |
30.59 |
29.62 |
-0.97 |
| 11 |
McFarland, Scott C (11)
|
NCWOW |
30.10 |
30.09 |
-0.01 |
| 12 |
Healey, Matthew J (11)
|
NCWOW |
30.36 |
30.52 |
0.16 |
| 13 |
Butner, Stephen A (11)
|
NCWOW |
31.17 |
31.05 |
-0.12 |
| 14 |
Aberant, Thomas A (12)
|
NCWAVE |
33.93 |
32.00 |
-1.93 |
| 15 |
Tompkins, Jonathan H (11)
|
NCWOW |
39.97 |
40.31 |
0.34 |
| 16 |
Baker, Mayes L (12)
|
NCWAVE |
|
40.97 |
- |
|
O'Malley, John C (11)
|
NCWOW |
|
NS |
- |