| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Adekoya, Oyinkansola O (10)
|
NCGCY |
40.87 |
40.11 |
-0.76 |
| 2 |
Wilkinson, Presley B (10)
|
NCTYDE |
42.25 |
41.28 |
-0.97 |
| 3 |
Vargas, Hanna S (10)
|
NCTYDE |
44.59 |
41.76 |
-2.83 |
| 4 |
Arrwood, Allison M (10)
|
SCRAYS |
46.52 |
44.22 |
-2.30 |
| 5 |
Owens, Hannah M (10)
|
NCLOY |
49.99 |
44.58 |
-5.41 |
| 6 |
Moore, Mary A (9)
|
NCTYDE |
43.23 |
44.74 |
1.51 |
| 7 |
Cinson, Alayna M (9)
|
NCYSST |
51.60 |
52.41 |
0.81 |
| 9 |
Bradham, Ava G (10)
|
NCTYDE |
55.16 |
59.22 |
4.06 |
| 10 |
Baird, Abigail N (9)
|
SCRAYS |
1:49.98 |
59.23 |
-50.75 |
| 11 |
Herlocker, Patterson L (9)
|
NCRACY |
1:01.87 |
1:02.40 |
0.53 |
| 12 |
Wheeler, Alyssa N (10)
|
NCTYDE |
1:04.68 |
1:06.05 |
1.37 |
| 13 |
Kelty, Lucy K (8)
|
NCYSST |
1:10.41 |
1:06.20 |
-4.21 |
| 14 |
Turowsky, Emily G (10)
|
SCYCSC |
1:23.35 |
1:12.52 |
-10.83 |
| 15 |
Hawkins, Charlotte J (7)
|
NCRACY |
1:28.99 |
1:18.15 |
-10.84 |
|
McKinney, Khloe D (8)
|
SCYCSC |
1:20.12 |
DQ |
- |
|
Cinson, Evelyn S (7)
|
NCYSST |
1:15.71 |
DQ |
- |
|
Ward, Maddison L (7)
|
NCYBAC |
1:38.10 |
DQ |
- |