| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Herron, Jayden S (17)
|
FLGSC |
|
47.75 |
- |
| 2 |
Becker, Sven W (14)
|
MNMAKO |
|
48.14 |
- |
| 3 |
Gallagher, Reece A (17)
|
SEMCC |
|
48.26 |
- |
| 4 |
Thionville, Pierce A (16)
|
LAWAVE |
|
48.30 |
- |
| 5 |
Crafton, Ryan J (18)
|
ILMAVS |
|
48.46 |
- |
| 6 |
Steen, Noah P (17)
|
CATORR |
|
48.94 |
- |
| 7 |
McLaren, Cael J (16)
|
ILACAD |
|
49.06 |
- |
| 8 |
Guerrero, Xander L (17)
|
ILSWAQ |
|
49.18 |
- |
| 9 |
Kelly, Andrew C (15)
|
ILWILD |
|
49.33 |
- |
| 10 |
Potter, Landon J (17)
|
SEBSC |
|
49.49 |
- |
| 11 |
Cumpston, Erik K (16)
|
VAPSDN |
|
49.58 |
- |
| 12 |
Blake, Joseph C (17)
|
NISTAR |
|
49.62 |
- |
| 13 |
Ramthun, John Cooper C (17)
|
MNMAKO |
|
49.71 |
- |
| 14 |
Ciolkosz, Ryan T (17)
|
WIMFSC |
|
50.04 |
- |
| 15 |
Sweat, Matthew T (16)
|
VAPSDN |
|
50.07 |
- |
| 16 |
Simic, Luka M (15)
|
ILSWAQ |
|
50.32 |
- |
|
Sandefer, Calvin D (16)
|
ILSSSC |
|
NS |
- |