| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Warnagiris, Kimberly E (15)
|
VAWST |
|
57.03 |
- |
| 2 |
Chetan, Nidhi (17)
|
ILFOX |
|
58.59 |
- |
| 3 |
Bowen, Brayden H (17)
|
MDNAAC |
|
58.63 |
- |
| 4 |
Dennis, Alyssa M (15)
|
SETNT |
|
58.85 |
- |
| 5 |
Tuttle, Annie D (17)
|
PVNCAP |
|
58.99 |
- |
| 6 |
Quinones, Melanie E (16)
|
ILWEST |
|
59.04 |
- |
| 7 |
Misenar, Morgan A (17)
|
SEBSC |
|
59.39 |
- |
| 8 |
Studebaker, Gabrielle W (18)
|
NISTAR |
|
59.40 |
- |
| 9 |
De Moya-Cotter, Gabriela T (16)
|
WIJ-HK |
|
59.78 |
- |
| 10 |
Holland, Elle C (18)
|
NITWST |
|
1:00.01 |
- |
| 11 |
Lumsden, Mollie J (15)
|
ILPPD |
|
1:00.32 |
- |
| 12 |
Makin, Emily M (15)
|
PVNCAP |
|
1:00.51 |
- |
| 13 |
Garcia, Aimee E (17)
|
CADSS |
|
1:00.55 |
- |
| 14 |
Perotti, Maria G (16)
|
PVMACH |
|
1:00.99 |
- |
| 15 |
Elliott, Maree E (16)
|
MNMAKO |
|
1:01.33 |
- |
| 16 |
Simila, Brooke M (16)
|
VARAYS |
|
1:02.04 |
- |