| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McKinney, Madison N (12)
|
NICLSC |
2:41.86 |
2:39.35 |
-2.51 |
| 2 |
Wagner, Morgan E (11)
|
NIVICT |
2:43.04 |
2:41.90 |
-1.14 |
| 3 |
Ramos, Hannah M (12)
|
NIRAYS |
2:44.20 |
2:43.58 |
-0.62 |
| 4 |
Prezyna, Zoey (12)
|
NIVICT |
2:51.90 |
2:44.17 |
-7.73 |
| 5 |
Ball, Lauryn R (12)
|
NIBRAD |
2:47.99 |
2:47.39 |
-0.60 |
| 6 |
Jokic, Sydney G (11)
|
NIWWSC |
2:54.74 |
2:53.12 |
-1.62 |
| 7 |
Puzanovova, Veronika A (11)
|
NIBAAC |
2:57.05 |
2:53.56 |
-3.49 |
| 8 |
Golden, Lauren B (11)
|
NIBAAC |
2:59.11 |
2:54.08 |
-5.03 |
| 9 |
Wamsley, Sarah E (12)
|
NIVELO |
2:54.43 |
2:56.50 |
2.07 |
| 10 |
Zagrobelny, Erin R (12)
|
NIEAST |
2:36.34 |
2:59.24 |
22.90 |
| 11 |
Noecker, Sophia E (12)
|
NITWST |
3:02.36 |
3:00.78 |
-1.58 |
| 12 |
Sheehan, Maura N (12)
|
NIGRAY |
3:03.50 |
3:03.84 |
0.34 |
| 13 |
Choroser, Penelope T (11)
|
NITWST |
2:47.40 |
3:07.18 |
19.78 |
| 14 |
Augustine, Sophie R (12)
|
NIVICT |
2:47.85 |
3:07.99 |
20.14 |
| 15 |
Maas, Kaylee A (11)
|
NIIRON |
2:48.81 |
3:09.32 |
20.51 |
| 16 |
Onze, Lauren W (12)
|
NIVICT |
2:34.16 |
3:10.73 |
36.57 |
| 17 |
Strittmatter, Amelia C (12)
|
NISTAR |
2:48.58 |
3:10.89 |
22.31 |
| 18 |
Philbrick, Mylie E (12)
|
NIVELO |
2:48.81 |
3:11.35 |
22.54 |
| 19 |
Scibetta, Madeline C (12)
|
NITTSC |
3:07.98 |
3:16.49 |
8.51 |
| 20 |
Barillari, Emma (12)
|
NIVELO |
2:47.21 |
3:19.88 |
32.67 |