| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barnhardt, Rylie D (14)
|
FGSFTL |
2:33.96 |
2:36.98 |
3.02 |
| 2 |
Perez, Meghan G (14)
|
FGGRSC |
2:36.82 |
2:38.41 |
1.59 |
| 3 |
MacVane, Riley C (14)
|
FGNPB |
2:40.42 |
2:39.68 |
-0.74 |
| 4 |
Kraft, Cassandra J (14)
|
FGGRSC |
2:33.95 |
2:41.89 |
7.94 |
| 5 |
Perez, Sophia E (14)
|
FGGRSC |
2:46.30 |
2:43.92 |
-2.38 |
| 6 |
Jorgensen, Kaitlyn D (13)
|
FGSUN |
2:44.28 |
2:46.27 |
1.99 |
| 7 |
Smith, Megan R (14)
|
FGSUN |
2:45.74 |
2:46.65 |
0.91 |
| 8 |
Renaud, Jade (13)
|
FGSF |
2:54.23 |
2:49.16 |
-5.07 |
| 9 |
McLemore, Tawannah P (13)
|
FGTSA |
|
2:49.33 |
- |
| 10 |
Estrada-Nunez, Thais I (14)
|
FGGRSC |
2:50.06 |
2:50.32 |
0.26 |
| 11 |
Freites, Andrea Federica (13)
|
FGAZFL |
|
2:51.17 |
- |
| 12 |
Leete, Siera A (14)
|
FGSFTL |
2:58.58 |
2:55.18 |
-3.40 |
| 13 |
Potts, Lindsay K (14)
|
FGBRST |
3:01.28 |
2:57.00 |
-4.28 |
| 14 |
Juskiewicz, Abigail E (13)
|
FGPST |
3:03.31 |
3:00.55 |
-2.76 |
| 15 |
Mirones, Cecilia (13)
|
FGSUN |
2:55.53 |
3:01.83 |
6.30 |
| 16 |
Gonzalez, Mya K (14)
|
FGMART |
3:04.59 |
3:06.59 |
2.00 |
| 17 |
Saballos, Fabiola (14)
|
FGPST |
3:22.98 |
3:26.90 |
3.92 |
| 18 |
Maharaj, Alina (14)
|
FGNPB |
|
3:31.07 |
- |
|
Sorunmu, Maya A (13)
|
FGSF |
2:50.97 |
NS |
- |
|
Lamelas, Sophie (14)
|
FGGRSC |
2:55.74 |
NS |
- |