| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Grannell, Merren A (12)
|
NTNTN |
31.44 |
33.58 |
2.14 |
| 2 |
McWhorter, Ella G (11)
|
NTLAC |
36.32 |
34.94 |
-1.38 |
| 3 |
Lofthus, Eva G (12)
|
NTLAC |
36.53 |
35.51 |
-1.02 |
| 4 |
Djerf, Ella M (12)
|
NTNTN |
34.82 |
36.09 |
1.27 |
| 5 |
Aegerter, Gabrielle A (11)
|
NTNTN |
39.50 |
36.26 |
-3.24 |
| 6 |
Backus, Lucy J (12)
|
NTLAC |
36.76 |
36.38 |
-0.38 |
| 7 |
Ho, Hannah V (12)
|
NTLAC |
37.47 |
36.47 |
-1.00 |
| 8 |
Feng, Emily Y (12)
|
NTNTN |
38.21 |
36.76 |
-1.45 |
| 9 |
Gupta, Ananya (11)
|
NTLAC |
36.60 |
37.07 |
0.47 |
| 10 |
Plows, Taryn S (11)
|
NTNTN |
39.74 |
37.13 |
-2.61 |
| 11 |
Thompson, Avery M (12)
|
NTLAC |
38.24 |
37.58 |
-0.66 |
| 11 |
Fox, Alexandra T (11)
|
NTUN |
39.24 |
37.58 |
-1.66 |
| 13 |
Schneider, Natalie M (11)
|
NTLAC |
36.85 |
38.51 |
1.66 |
| 14 |
Gutierrez, Luna (11)
|
NTNTN |
39.99 |
38.83 |
-1.16 |
| 15 |
McNeeley, Chloe Marie Y (12)
|
NTLAC |
39.00 |
39.36 |
0.36 |
|
Colombo, Olivia C (11)
|
NTNTN |
36.22 |
NS |
- |