| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Witte, Griffin E (17)
|
NIWEBS |
54.50 |
57.33 |
2.83 |
| 2 |
Murphy, Liam (16)
|
NIPACK |
59.27 |
57.91 |
-1.36 |
| 3 |
Jacobs, Bradford C (17)
|
NIPACK |
56.33 |
58.69 |
2.36 |
| 4 |
Kuppel, Brandon A (18)
|
NITWST |
1:00.71 |
1:01.12 |
0.41 |
| 5 |
Wangelin, Charlie L (15)
|
NITWST |
1:03.81 |
1:01.27 |
-2.54 |
| 6 |
Adams, Marshall B (13)
|
NITWST |
1:02.40 |
1:01.55 |
-0.85 |
| 7 |
Palmer, Alexander R (13)
|
NIFAST |
1:06.06 |
1:02.52 |
-3.54 |
| 8 |
Hahn, Kurt C (14)
|
NIWEBS |
1:03.45 |
1:05.25 |
1.80 |
| 9 |
Low, Casey M (15)
|
NIHAMB |
1:04.09 |
1:05.37 |
1.28 |
| 10 |
Markham, Rowan M (16)
|
NIFAST |
1:05.49 |
1:05.96 |
0.47 |
| 11 |
Bedford, Keegan L (14)
|
NIWEBS |
1:07.25 |
1:07.46 |
0.21 |
| 12 |
Kjellstrom, Anders J (13)
|
NITWST |
1:09.57 |
1:08.42 |
-1.15 |
| 13 |
Weiner, Lucas M (14)
|
NIFAST |
1:07.52 |
1:08.45 |
0.93 |
| 14 |
Nenadic, Alexander B (15)
|
NIFAST |
1:08.95 |
1:10.44 |
1.49 |
| 15 |
Webb, Douglas J (15)
|
NIFAST |
1:09.50 |
1:10.97 |
1.47 |
|
Kibler, Seth A (16)
|
NITWST |
56.38 |
NS |
- |
|
McCrone, Patrick (15)
|
NITWST |
59.84 |
NS |
- |