| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Puram, Harshitha * (17)
|
OHGYG |
27.70 |
27.88 |
0.18 |
| 2 |
Braun, Taylor O (13)
|
OHGYG |
27.84 |
28.03 |
0.19 |
| 3 |
Basham, Katelyn N (13)
|
OHHURY |
29.84 |
29.03 |
-0.81 |
| 4 |
Lowrie, Ella F (13)
|
OHLTPY |
28.33 |
29.32 |
0.99 |
| 5 |
Harrison-Mills, Mya L (13)
|
OHLTPY |
29.20 |
29.51 |
0.31 |
| 6 |
Martin, Brynn A (13)
|
OHHURY |
29.66 |
30.27 |
0.61 |
| 7 |
Mora, Julia R (13)
|
OHEAST |
30.84 |
30.76 |
-0.08 |
| 8 |
Scolnick, Allison T (14)
|
OHGYG |
30.89 |
30.98 |
0.09 |
| 9 |
Miller, Lillian M (13)
|
OHLTPY |
32.06 |
32.02 |
-0.04 |
| 10 |
McClurg, Carlie P (13)
|
OHLYST |
32.86 |
32.18 |
-0.68 |
| 11 |
Monhollen, Carley A (13)
|
OHEAST |
31.40 |
33.13 |
1.73 |
| 12 |
Anderson, Megan R (13)
|
OHLTPY |
31.90 |
33.35 |
1.45 |
| 13 |
Hutton, Zelma (14)
|
OHCHY |
30.90 |
33.58 |
2.68 |
| 14 |
Stewart, Ellison J (13)
|
OHHURY |
33.68 |
33.88 |
0.20 |
| 15 |
Brenner, Kerigan A (13)
|
OHMCY |
34.79 |
33.94 |
-0.85 |
| 16 |
Pollock, Addison R (13)
|
OHHURY |
33.38 |
34.03 |
0.65 |
| 17 |
Nollenberger, Molly A (13)
|
OHLTPY |
36.58 |
35.40 |
-1.18 |
| 18 |
Peruri, Tanvi P (13)
|
OHLTPY |
37.88 |
36.32 |
-1.56 |
| 19 |
Gudla, Sankeertana * (13)
|
OHLTPY |
39.07 |
36.84 |
-2.23 |
| 20 |
Seth, Shivani * (13)
|
OHLTPY |
37.80 |
40.48 |
2.68 |
|
Uthup, Marissa * (13)
|
OHLTPY |
44.84 |
NS |
- |