| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kelley, William G (17)
|
OHCY |
22.95 |
23.50 |
0.55 |
| 2 |
Furuta, Roman R (15)
|
OHRCDB |
23.42 |
23.99 |
0.57 |
| 3 |
Richter, Camden J (16)
|
OHMCY |
24.96 |
24.35 |
-0.61 |
| 4 |
Smal, Frederik F (16)
|
OHRCDB |
24.33 |
24.38 |
0.05 |
| 5 |
Eshler, Nathan A (18)
|
OHCY |
24.72 |
25.27 |
0.55 |
| 6 |
Perry, Max S (15)
|
OHRCDB |
26.83 |
26.48 |
-0.35 |
| 7 |
Hutcherson, Josiah C (15)
|
OHMCY |
27.18 |
26.82 |
-0.36 |
| 8 |
Christenson, Matthew D (15)
|
OHMCY |
26.56 |
26.90 |
0.34 |
| 9 |
Coop, Parker (15)
|
OHRCDB |
|
27.21 |
- |
| 10 |
Barnett, Matthew D (15)
|
OHCY |
33.37 |
29.67 |
-3.70 |
|
Combs, Aidan W (17)
|
OHCY |
26.50 |
NS |
- |