| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rabah, Luana (9)
|
PVASA |
43.10 |
41.20 |
-1.90 |
| 2 |
Hirsch, Mikayla B (9)
|
PVNCAP |
48.43 |
42.54 |
-5.89 |
| 3 |
Valdecanas, Savannah E (9)
|
PVHACC |
|
42.93 |
- |
| 4 |
Cota-Robles, Isabella V (9)
|
PVMACH |
|
45.08 |
- |
| 5 |
Na, Audrey G (9)
|
PVNCAP |
46.98 |
45.85 |
-1.13 |
| 6 |
Stadelmann, Emma (9)
|
PVNCAP |
|
46.23 |
- |
| 7 |
Walters, Lila M (9)
|
PVAAC |
|
47.28 |
- |
| 8 |
Lay, Sydney C (9)
|
PVMACH |
|
47.51 |
- |
| 9 |
Peter, Noelle (9)
|
PVHACC |
|
49.52 |
- |
| 10 |
Agbomson, Elizabeth N (9)
|
PVMACH |
|
49.79 |
- |
| 11 |
Katsaros, Charlotte M (9)
|
PVASA |
53.05 |
50.40 |
-2.65 |
| 12 |
Gilbert, Anne G (9)
|
PVASA |
|
51.37 |
- |
| 13 |
Schimmel, Emily A (9)
|
PVAAC |
|
51.41 |
- |
| 14 |
Pickard, Abigail J (9)
|
PVMACH |
|
53.40 |
- |
| 15 |
Waller, Elliana S (9)
|
PVFBST |
50.02 |
55.72 |
5.70 |
| 16 |
Boardman, Taylor B (9)
|
PVHACC |
|
56.52 |
- |
| 17 |
McFarren, Riley J (9)
|
PVAAC |
1:09.87 |
1:03.40 |
-6.47 |
| 18 |
Gomez Crummett, Candela N (9)
|
PVNCAP |
|
1:05.23 |
- |
|
Diaz-Lujan, Isabella M (9)
|
PVNCAP |
|
DQ |
- |
|
Fuelling, Addison L (9)
|
PVNCAP |
|
DQ |
- |
|
Biso-Aponte, Katarina E (9)
|
PVAAC |
|
DQ |
- |
|
Logsdon, Evelyn E (9)
|
PVAAC |
|
NS |
- |
|
Epstein, Lila L (9)
|
PVNCAP |
|
NS |
- |