| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lucchese, Alexandra G (8)
|
PVNCAP |
36.17 |
35.63 |
-0.54 |
| 2 |
Harms, Stella J (8)
|
PVNCAP |
37.95 |
35.68 |
-2.27 |
| 3 |
Routman, Kelsey A (8)
|
PVBWST |
40.27 |
40.38 |
0.11 |
| 4 |
Shoemaker, Madeline G (8)
|
PVNCAP |
39.64 |
40.90 |
1.26 |
| 5 |
Olivo Cauberghs, Amaya J (8)
|
PVNCAP |
41.04 |
41.49 |
0.45 |
| 6 |
Zhao, Alice R (8)
|
PVNCAP |
44.24 |
43.15 |
-1.09 |
| 7 |
Huang, Iliza Q (8)
|
PVNCAP |
|
43.45 |
- |
| 8 |
Jung, Alina Y (8)
|
PVNCAP |
47.30 |
44.90 |
-2.40 |
| 9 |
Burton, Avery C (8)
|
PVBWST |
46.06 |
45.63 |
-0.43 |
| 10 |
Tapia, Liliana G (8)
|
PVNCAP |
|
46.35 |
- |
| 11 |
Burt, Emmalyne-Grace J (8)
|
PVBWST |
48.08 |
47.11 |
-0.97 |
| 12 |
Duffy, Susannah G (8)
|
PVNCAP |
45.01 |
48.01 |
3.00 |
| 13 |
Nugent, Emily J (8)
|
PVNCAP |
|
50.15 |
- |
| 14 |
Richards, Ella M (8)
|
PVNCAP |
53.87 |
50.69 |
-3.18 |
| 15 |
Rosenthal, Chloe J (8)
|
PVNCAP |
48.75 |
51.04 |
2.29 |
| 16 |
Gholson, Grace E (8)
|
PVBWST |
|
51.44 |
- |
| 17 |
Reed, Simone M (8)
|
PVNCAP |
|
51.56 |
- |
| 18 |
Schmitz, Jordyn T (8)
|
PVBWST |
53.52 |
57.63 |
4.11 |
|
Morrissette, Madeleine V (8)
|
PVNCAP |
|
NS |
- |