| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Anspach, Ayanna (8)
|
PVTIBU |
26.02 |
23.04 |
-2.98 |
| 2 |
Lewis, Jillian A (8)
|
PVTIBU |
25.33 |
24.87 |
-0.46 |
| 3 |
Saarinen, Cecilia B (8)
|
PVPAC |
27.26 |
26.28 |
-0.98 |
| 4 |
Smith, Marley A (8)
|
PVLIFE |
37.30 |
27.84 |
-9.46 |
| 5 |
Hughes, Emerson E (8)
|
PVPAC |
31.07 |
31.79 |
0.72 |
| 6 |
Ibrahim-Qayyum, Aniya Z (8)
|
PVTIBU |
33.04 |
32.13 |
-0.91 |
| 7 |
Batty, Leah M (8)
|
PVPAC |
30.90 |
32.49 |
1.59 |
| 8 |
Peterson Delaney, Fiona R (8)
|
PVPAC |
34.81 |
34.06 |
-0.75 |
| 9 |
Peterson Delaney, Ella J (8)
|
PVPAC |
36.99 |
34.55 |
-2.44 |
| 10 |
Martin, Alexandra B (8)
|
PVPGPR |
48.20 |
43.88 |
-4.32 |