| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Egan, Margaret M (9)
|
PVDCPR |
35.26 |
34.43 |
-0.83 |
| 2 |
Pascuccio Gomez, Fiona (10)
|
PVDCPR |
37.48 |
36.59 |
-0.89 |
| 3 |
Minalu, Kalkidan A (9)
|
PVDCPR |
40.78 |
40.21 |
-0.57 |
| 4 |
Beggiato, Eva M (10)
|
PVDCPR |
38.70 |
41.13 |
2.43 |
| 5 |
White, Tanji C (10)
|
PVDCPR |
|
42.37 |
- |
| 6 |
Johnson, Jasmine L (9)
|
PVDCPR |
40.30 |
43.80 |
3.50 |
| 7 |
Alston, Carley M (9)
|
PVDCPR |
47.45 |
45.98 |
-1.47 |
| 8 |
Johnston, Abigail H (9)
|
PVDCPR |
44.04 |
46.82 |
2.78 |
| 9 |
Hernandez, Lula M (9)
|
PVDCPR |
|
53.11 |
- |
| 10 |
Bunn, Logan M (9)
|
PVDCPR |
53.60 |
56.05 |
2.45 |
|
Raspberry-Hallmon, Riley S (10)
|
PVDCPR |
48.34 |
NS |
- |