| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeBaecke, Sydney E (14)
|
MADST |
5:09.72 |
5:17.59 |
7.87 |
| 1 |
Brown, Hailey G (13)
|
PVFISH |
6:21.23 |
5:31.27 |
-49.96 |
| 2 |
O'Shaughnessy, Bridget E (14)
|
PVFISH |
5:18.12 |
5:21.19 |
3.07 |
| 2 |
Creeks, Sofia A (13)
|
PVFISH |
6:01.01 |
5:58.57 |
-2.44 |
| 3 |
Colborn, Georgia A (13)
|
MADST |
5:36.60 |
5:23.02 |
-13.58 |
| 3 |
Shields, Ashleigh N (14)
|
MADST |
6:06.76 |
6:15.32 |
8.56 |
| 4 |
Yang, Georgia M (14)
|
PVASA |
5:25.01 |
5:24.55 |
-0.46 |
| 4 |
Savage, Meghan N (14)
|
PVFISH |
6:11.11 |
6:20.92 |
9.81 |
| 5 |
Fitzgerald, Katelyn G (14)
|
MADST |
5:49.20 |
5:26.83 |
-22.37 |
| 5 |
Guthrie, Sydney E (13)
|
PVFISH |
6:10.10 |
6:36.33 |
26.23 |
| 6 |
Keegan, Mary K (14)
|
MADST |
5:39.55 |
5:28.92 |
-10.63 |
| 7 |
Horning, Caitlin L (13)
|
MADST |
5:13.62 |
5:30.39 |
16.77 |
| 8 |
Memon, Zoya N (13)
|
PVASA |
5:30.75 |
5:30.95 |
0.20 |
| 9 |
Iwasyk, Izzy B (13)
|
MADST |
5:27.82 |
5:33.53 |
5.71 |
| 10 |
Boughanem, Hanna Y (14)
|
PVFISH |
5:42.07 |
5:33.97 |
-8.10 |
| 11 |
Brindle, Devin R (14)
|
PVASA |
5:59.34 |
5:46.16 |
-13.18 |
| 12 |
Cronin, Mealy H (13)
|
PVFISH |
5:54.54 |
6:02.53 |
7.99 |