| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sherman, Lila I (10)
|
PVFISH |
3:28.54 |
3:03.18 |
-25.36 |
| 1 |
Fleming, SamanthaJo J (10)
|
PVFISH |
3:45.42 |
3:14.50 |
-30.92 |
| 2 |
Kiess, Andie N (9)
|
MADST |
3:19.13 |
3:05.16 |
-13.97 |
| 2 |
Bostian, Sophia M (10)
|
MDFCY |
4:15.39 |
4:11.91 |
-3.48 |
| 3 |
O'Shaughnessy, Anne M (9)
|
PVFISH |
3:34.38 |
3:27.23 |
-7.15 |
| 4 |
Bendza, Corinne J (8)
|
PVFISH |
3:21.11 |
3:35.24 |
14.13 |
| 5 |
Anderson, Trinity A (10)
|
MDHFY |
3:05.58 |
3:40.29 |
34.71 |
| 6 |
Roca, Olivia E (10)
|
PVMAKO |
3:13.08 |
3:42.98 |
29.90 |
| 7 |
Moyer, Kayli R (10)
|
MDHFY |
3:05.78 |
3:44.50 |
38.72 |
| 8 |
Proctor, Rainaka R (10)
|
PVDCPR |
3:41.27 |
3:48.10 |
6.83 |
| 9 |
Peters, Riley S (10)
|
PVFISH |
3:38.88 |
4:02.34 |
23.46 |