| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Armstrong, Katelyn A (10)
|
PVMACH |
43.83 |
43.06 |
-0.77 |
| 2 |
Chow, Chloe S (10)
|
PVMACH |
45.98 |
46.55 |
0.57 |
| 3 |
Burton, Bryce L (10)
|
PVMACH |
46.91 |
47.23 |
0.32 |
| 4 |
Joffee, Nora H (10)
|
PVMACH |
48.33 |
47.42 |
-0.91 |
| 5 |
McCroskey, Madeleine E (10)
|
PVSTJS |
49.91 |
47.52 |
-2.39 |
| 6 |
Noojipady, Meghana L (10)
|
PVMACH |
1:00.24 |
47.81 |
-12.43 |
| 7 |
Kang, Stella D (10)
|
PVMACH |
51.07 |
49.93 |
-1.14 |
| 8 |
Marquardt, Alia M (10)
|
PVMACH |
49.35 |
50.42 |
1.07 |
| 9 |
Zheng, Emma A (10)
|
PVMACH |
|
53.11 |
- |
| 10 |
Amirullah, Faiza Q (10)
|
PVMACH |
58.30 |
55.34 |
-2.96 |
| 11 |
Wright, Tessa E (10)
|
PVSTJS |
|
58.61 |
- |
| 12 |
Backus, Helena V (10)
|
PVMACH |
1:00.01 |
1:01.06 |
1.05 |
| 13 |
Cunningham, Whitney A (10)
|
PVMACH |
|
1:04.76 |
- |
|
McCarthy, Kayleigh M (10)
|
PVMACH |
59.06 |
NS |
- |
|
Portella, Cara A (10)
|
PVMACH |
52.79 |
NS |
- |
|
Rivera, Rachel S (10)
|
PVMACH |
53.46 |
NS |
- |
|
Dixon, Lordon (10)
|
PVSTJS |
53.39 |
NS |
- |