| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
King, Anna M (9)
|
PVMACH |
41.03 |
40.48 |
-0.55 |
| 2 |
Vazquez, Lucia B (9)
|
PVSTJS |
42.35 |
41.59 |
-0.76 |
| 3 |
Kent, Elizabeth S (9)
|
PVSTJS |
45.83 |
42.24 |
-3.59 |
| 4 |
Sarathy, Anoushka (9)
|
PVMACH |
44.30 |
42.76 |
-1.54 |
| 5 |
Cota-Robles, Isabella V (9)
|
PVMACH |
43.97 |
46.55 |
2.58 |
| 6 |
Goudie, Annabelle L (9)
|
PVMACH |
44.39 |
47.21 |
2.82 |
| 7 |
Charles-Williamson, Ssanyu N (9)
|
PVMACH |
50.72 |
47.55 |
-3.17 |
| 8 |
Bayer, Jillian R (9)
|
PVMACH |
|
47.81 |
- |
| 9 |
Waas, Maeve S (9)
|
PVMACH |
|
48.10 |
- |
| 10 |
Newgen, Kaylee S (9)
|
PVMACH |
48.71 |
50.34 |
1.63 |
| 11 |
Lee, Julia V (9)
|
PVMACH |
52.95 |
53.65 |
0.70 |
| 12 |
Detrisac, Marissa R (9)
|
PVMACH |
59.34 |
59.54 |
0.20 |
| 13 |
Conley, Keira E (9)
|
PVSTJS |
|
1:11.34 |
- |
|
Rivera-Castro, Noelle I (9)
|
PVMACH |
|
DQ |
- |
|
Abel, Lara A (9)
|
PVSTJS |
|
DQ |
- |
|
Dean, Megan E (9)
|
PVMACH |
|
DQ |
- |
|
Bayer, Amelia J (9)
|
PVMACH |
|
DQ |
- |
|
Bagley, Kate S (9)
|
PVMACH |
|
NS |
- |
|
Harrell, Sloane B (9)
|
PVMACH |
52.37 |
NS |
- |
|
Berry, Caroline J (9)
|
PVSTJS |
|
NS |
- |