| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fredericks, Sophie C (11)
|
PVMACH |
35.81 |
40.65 |
4.84 |
| 2 |
Emery, Norah L (12)
|
PVPM |
42.87 |
40.99 |
-1.88 |
| 3 |
Sims, Marisol W (12)
|
PVSNOW |
42.00 |
42.18 |
0.18 |
| 4 |
Pezzimenti, Alessandra E (12)
|
PVMACH |
35.34 |
42.71 |
7.37 |
| 5 |
Gross, Caitlin M (12)
|
PVMACH |
38.82 |
42.75 |
3.93 |
| 6 |
Ismail, Mariam O (11)
|
PVSNOW |
47.05 |
44.72 |
-2.33 |
| 7 |
Kass, Morgan L (11)
|
PVMACH |
39.64 |
45.50 |
5.86 |
| 8 |
Ruschaupt, Olivia C (12)
|
PVSNOW |
44.89 |
45.72 |
0.83 |
| 9 |
McCurry, Eva M (12)
|
PVSNOW |
44.04 |
46.12 |
2.08 |
| 10 |
Poulos, Paige A (11)
|
PVNCAP |
45.42 |
47.02 |
1.60 |
| 11 |
Gleditsch, Caroline M (11)
|
PVNCAP |
42.98 |
47.13 |
4.15 |
| 12 |
Redash, Natalie S (12)
|
PVSNOW |
47.18 |
47.45 |
0.27 |
| 13 |
Althouse, Anna N (11)
|
PVPM |
50.36 |
47.73 |
-2.63 |
| 14 |
Zenyuh, Helena M (11)
|
PVPM |
49.78 |
47.90 |
-1.88 |
| 15 |
Meka, Sanika (11)
|
PVNCAP |
49.53 |
48.73 |
-0.80 |
| 16 |
Asner, Adira M (12)
|
PVMACH |
42.37 |
48.84 |
6.47 |
| 17 |
Porter, Drew A (11)
|
PVMACH |
42.79 |
50.02 |
7.23 |
| 18 |
Crapco-Wang, Caitlyn L (12)
|
PVMACH |
41.67 |
50.89 |
9.22 |
| 19 |
Nahid, Heidi (12)
|
PVNCAP |
48.79 |
52.01 |
3.22 |
| 20 |
McAllister, Maia C (11)
|
PVPM |
51.00 |
55.96 |
4.96 |
|
Feehs, Maryn Y (12)
|
PVPM |
45.54 |
NS |
- |
|
Lee-Truesdell, Isabella S (12)
|
PVMACH |
40.59 |
NS |
- |
|
Janczyk, Megan D (12)
|
PVMACH |
34.69 |
NS |
- |
|
Bordovsky, Abigail L (11)
|
PVMACH |
40.89 |
NS |
- |