| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bui, Emma T (8)
|
PVMACH |
41.73 |
41.44 |
-0.29 |
| 2 |
Miller, McKenzie L (8)
|
PVMACH |
42.93 |
42.42 |
-0.51 |
| 3 |
King, Anna M (8)
|
PVMACH |
40.81 |
44.14 |
3.33 |
| 4 |
Dressendorfer, Beatrice L (8)
|
PVMACH |
49.38 |
44.64 |
-4.74 |
| 5 |
Dressendorfer, Carson E (8)
|
PVMACH |
45.12 |
45.06 |
-0.06 |
| 6 |
Malinowski, Sophia N (8)
|
PVNCAP |
|
46.03 |
- |
| 7 |
Sarathy, Anoushka (8)
|
PVMACH |
47.29 |
48.15 |
0.86 |
| 8 |
Cypher, Fiona S (8)
|
PVNCAP |
48.97 |
48.73 |
-0.24 |
| 9 |
Schroeder, Elizabeth C (8)
|
PVNCAP |
51.51 |
49.78 |
-1.73 |
| 10 |
Wittmer, Grace K (8)
|
PVPM |
54.80 |
51.57 |
-3.23 |
| 11 |
Cho, Olivia Y (7)
|
PVMACH |
54.59 |
52.26 |
-2.33 |
| 12 |
Townsend, Caitlin D (7)
|
PVPM |
1:00.63 |
55.26 |
-5.37 |
| 13 |
Ward, Hayden D (8)
|
PVPM |
|
55.91 |
- |
| 14 |
Cho, Claire Y (7)
|
PVMACH |
1:00.17 |
56.74 |
-3.43 |
| 15 |
Demissie, Hannah B (7)
|
PVNCAP |
|
58.86 |
- |
| 16 |
Don, Audrey H (8)
|
PVPM |
1:01.33 |
59.65 |
-1.68 |
| 17 |
Anderson, Emma E (8)
|
PVPM |
56.34 |
1:01.99 |
5.65 |
| 18 |
Gunter, June E (8)
|
PVPM |
1:02.90 |
1:04.48 |
1.58 |
| 19 |
Branham, Maeve E (6)
|
PVMACH |
1:21.52 |
1:15.89 |
-5.63 |
|
Penetrante, Leia M (8)
|
PVPM |
49.44 |
DQ |
- |
|
Yu, Sophia G (7)
|
PVMACH |
56.02 |
NS |
- |