| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Orlic, Xavier J (14)
|
PVUN |
54.01 |
53.80 |
-0.21 |
| 2 |
Kim, Junwoo C (14)
|
PVNCAP |
56.27 |
53.89 |
-2.38 |
| 3 |
Nurkic Kacapor, Mak E (14)
|
PVNCAP |
55.55 |
54.18 |
-1.37 |
| 4 |
Mallios, Braden G (14)
|
PVNCAP |
56.93 |
56.16 |
-0.77 |
| 5 |
Robinson, Noah K (13)
|
PVNCAP |
57.33 |
56.46 |
-0.87 |
| 6 |
Bacon, Finn E (13)
|
PVNCAP |
58.85 |
57.80 |
-1.05 |
| 7 |
McDonald, Jonah S (14)
|
PVDCPR |
1:01.93 |
59.78 |
-2.15 |
| 8 |
Harrison, Joshua D (14)
|
PVNCAP |
1:03.28 |
1:00.42 |
-2.86 |
| 9 |
Sullivan, Nathan E (13)
|
PVNCAP |
1:00.00 |
1:00.92 |
0.92 |
| 10 |
Wallace, Nathaniel J (13)
|
PVDCPR |
1:02.69 |
1:02.67 |
-0.02 |