| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Campbell, Sydney K (8)
|
PVMSSC |
41.13 |
40.53 |
-0.60 |
| 2 |
Fox, Chloe (8)
|
PVYASD |
51.30 |
52.73 |
1.43 |
| 3 |
Anspach, Ayanna (8)
|
PVTIBU |
|
54.57 |
- |
| 5 |
Montilla, Jocelyn A (8)
|
PVPM |
52.27 |
55.40 |
3.13 |
| 6 |
Young, Jessica Z (8)
|
PVFISH |
|
56.68 |
- |
| 7 |
Almeida, Veronica (8)
|
PVPM |
59.34 |
59.46 |
0.12 |
| 8 |
Weliwitigoda, Mila A (7)
|
PVNCAP |
|
1:01.21 |
- |
| 9 |
Zheng, Evelyn M (8)
|
PVFISH |
1:00.05 |
1:01.48 |
1.43 |
| 10 |
Nogales, Allegra K (7)
|
PVFISH |
1:01.93 |
1:02.17 |
0.24 |
| 11 |
Faltemier, Abigail L (8)
|
PVPM |
1:05.86 |
1:04.05 |
-1.81 |
| 12 |
Himmelsteib, Melissa E (8)
|
PVFISH |
1:06.49 |
1:04.40 |
-2.09 |
| 13 |
Estes, Lulu F (6)
|
PVPM |
1:11.37 |
1:06.28 |
-5.09 |
| 14 |
Evans, Khloe L (8)
|
PVPAC |
|
1:07.14 |
- |
| 15 |
Greenfield, Jordan D (8)
|
PVFISH |
1:06.35 |
1:07.18 |
0.83 |
| 16 |
Bayona, Laura A (8)
|
PVMSSC |
1:08.96 |
1:07.32 |
-1.64 |
| 17 |
Dmitrova, Varvara (7)
|
PVFISH |
|
1:07.57 |
- |
| 18 |
Bice, Annabella Y (8)
|
PVPAC |
|
1:10.38 |
- |
| 19 |
Hixson, Hannah Y (8)
|
PVPM |
|
1:14.22 |
- |
|
Netherton, Scarlett M (7)
|
PVPAC |
|
DQ |
- |
|
Chennamsetti, Karina D (8)
|
PVTIBU |
|
DQ |
- |