| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sislen, Leonardo S (11)
|
PVNCAP |
34.70 |
34.44 |
-0.26 |
| 1 |
Rojas, Matteo N (12)
|
MDFCY |
39.53 |
35.45 |
-4.08 |
| 1 |
Olivo Cauberghs, Esteban C (11)
|
PVNCAP |
37.17 |
42.04 |
4.87 |
| 2 |
Kleczek, Antoni I (12)
|
PVUN |
33.87 |
34.79 |
0.92 |
| 2 |
Das, Rohit G (12)
|
PVNCAP |
38.18 |
39.03 |
0.85 |
| 2 |
Vorozheykin, Andrew (11)
|
PVNCAP |
39.57 |
43.10 |
3.53 |
| 3 |
Reeves, John N (12)
|
PVNCAP |
40.13 |
39.95 |
-0.18 |
| 3 |
Rankin, Cavan R (12)
|
MDFCY |
44.54 |
45.05 |
0.51 |
| 4 |
Garcia, Chase A (12)
|
VATORP |
38.44 |
41.32 |
2.88 |
| 4 |
Zhao, Andrew (11)
|
PVNCAP |
42.26 |
45.32 |
3.06 |
| 6 |
Kaleem, Jacob D (12)
|
PVNCAP |
45.37 |
48.88 |
3.51 |
| 7 |
Lightfoot, William A (11)
|
PVERSC |
51.94 |
59.48 |
7.54 |
| 8 |
Nguyen, Carson N (12)
|
VATORP |
1:00.65 |
1:03.48 |
2.83 |
|
Xu, Kevin (12)
|
PVPAC |
|
DQ |
- |
|
Belen, Alfonso M (12)
|
PVERSC |
44.82 |
NS |
- |
|
Mettler, Trent C (12)
|
PVTIBU |
31.90 |
NS |
- |