| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shirk, Brianna M (11)
|
VAWST |
41.28 |
39.87 |
-1.41 |
| 2 |
Snowden, Viktoria E (9)
|
PVSNOW |
42.14 |
43.33 |
1.19 |
| 3 |
Shah, Aanya B (10)
|
PVBWST |
47.00 |
43.77 |
-3.23 |
| 3 |
McCormick, Norah H (11)
|
PVSNOW |
46.97 |
43.77 |
-3.20 |
| 5 |
Matta, Nekeisha (12)
|
PVSNOW |
45.00 |
43.89 |
-1.11 |
| 6 |
Vanek, Hadley E (10)
|
PVNCAP |
45.62 |
45.36 |
-0.26 |
| 7 |
Vizcaino, Lyra D (9)
|
PVSNOW |
51.89 |
46.15 |
-5.74 |
| 8 |
Woehr, Allison E (10)
|
PVSNOW |
45.43 |
47.98 |
2.55 |
| 9 |
Ji, Rachel Y (10)
|
PVUN-M |
49.66 |
48.00 |
-1.66 |
| 10 |
DeLong, Abigail R (8)
|
PVBWST |
54.70 |
49.59 |
-5.11 |
| 11 |
Jonnalagadda, Sahasra A (11)
|
PVBWST |
56.27 |
49.72 |
-6.55 |
| 12 |
Trivedi, Samya D (10)
|
PVNCAP |
53.50 |
51.58 |
-1.92 |
| 13 |
Swanson, Madeline E (11)
|
PVSNOW |
52.58 |
52.00 |
-0.58 |
| 14 |
Lynch, Paige T (10)
|
PVSNOW |
51.61 |
52.03 |
0.42 |
| 15 |
Arekal, Adya N (8)
|
PVBWST |
52.56 |
53.72 |
1.16 |
| 16 |
Thor, GaoHli A (8)
|
PVSNOW |
50.13 |
55.03 |
4.90 |
| 17 |
Alic, Hanna (10)
|
PVBWST |
1:01.42 |
57.40 |
-4.02 |
|
Regal, Isabella (9)
|
PVBWST |
1:00.84 |
DQ |
- |
|
Gunnerson-Casca, Christina M (9)
|
PVBWST |
59.11 |
NS |
- |