| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
In, Emily (10)
|
PVMAC |
40.65 |
40.41 |
-0.24 |
| 2 |
Orimolade, Kendall J (10)
|
PVFAST |
43.85 |
44.08 |
0.23 |
| 3 |
Pineiro, Carolina S (9)
|
PVWSH |
|
44.46 |
- |
| 4 |
Hutchcroft, Paige F (10)
|
PVPAC |
45.99 |
46.24 |
0.25 |
| 5 |
Ji, Rachel Y (10)
|
PVWSH |
44.89 |
47.14 |
2.25 |
| 6 |
Downs, Mia B (10)
|
PVFAST |
44.05 |
47.60 |
3.55 |
| 7 |
Campos, Isabel S (9)
|
PVDCPR |
52.51 |
48.11 |
-4.40 |
| 8 |
Patel, Sarisha C (10)
|
PVDCPR |
52.15 |
51.20 |
-0.95 |
| 9 |
Robinson, Alyssa C (8)
|
PVMSSC |
46.29 |
52.31 |
6.02 |
| 10 |
Beggiato, Eva M (10)
|
PVDCPR |
51.75 |
53.34 |
1.59 |
| 11 |
Batty, Leah M (9)
|
PVPAC |
49.84 |
56.53 |
6.69 |
| 12 |
Jefferson, Alexis N (10)
|
PVDCPR |
53.61 |
59.08 |
5.47 |
| 13 |
Johnston, Abigail H (9)
|
PVDCPR |
|
1:00.76 |
- |
| 14 |
Ashurst, Kaya (9)
|
PVFAST |
1:03.31 |
1:03.74 |
0.43 |
| 15 |
Francis, Molly R (7)
|
PVPAC |
|
1:16.23 |
- |
| 16 |
Sherrod, Savannah G (9)
|
PVPAC |
1:12.52 |
1:33.97 |
21.45 |
|
Titherington, Harper J (9)
|
PVDCPR |
|
DQ |
- |
|
Raspberry-Hallmon, Riley S (10)
|
PVDCPR |
|
DQ |
- |
|
Davis, Violet E (8)
|
PVDCPR |
|
DQ |
- |
|
Buckner, Zora N (8)
|
PVDCPR |
|
DQ |
- |
|
Beggiato, Aria L (7)
|
PVDCPR |
|
DQ |
- |
|
Soloway, Lucie J (9)
|
PVDCPR |
1:04.85 |
NS |
- |
|
Martin, Alexandra B (9)
|
PVDCPR |
|
NS |
- |
|
Smith, Camille L (10)
|
PVFAST |
44.98 |
NS |
- |