| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Xin, Jason (10)
|
PVDRAG |
42.60 |
39.77 |
-2.83 |
| 2 |
Harris, Nicholas C (9)
|
PVPM |
41.10 |
39.99 |
-1.11 |
| 3 |
Zenyuh, Quinn R (9)
|
PVPM |
42.17 |
40.56 |
-1.61 |
| 4 |
Miceli, Jack M (10)
|
PVBWST |
40.49 |
41.17 |
0.68 |
| 5 |
van de Graaff, Anthony D (10)
|
PVMAKO |
42.78 |
43.39 |
0.61 |
| 6 |
Estes, Jasper L (10)
|
PVPM |
44.34 |
43.47 |
-0.87 |
| 7 |
Wang, Allen Y (10)
|
PVDRAG |
43.88 |
43.61 |
-0.27 |
| 8 |
Bravery, Jackson H (9)
|
PVCSC |
45.89 |
44.19 |
-1.70 |
| 9 |
Mourad, Ibrahim M (9)
|
PVPM |
48.60 |
44.77 |
-3.83 |
| 10 |
Minear, Connor M (9)
|
PVAAC |
42.19 |
45.21 |
3.02 |
| 11 |
Song, Joshua J (10)
|
PVBWST |
45.70 |
46.25 |
0.55 |
| 12 |
Gruendel, Josef T (10)
|
PVMAKO |
44.16 |
46.54 |
2.38 |
| 13 |
Schuyler, Matthew L (10)
|
PVFAST |
|
48.53 |
- |
| 14 |
Sneed, Calvin R (9)
|
PVPM |
|
53.91 |
- |
| 15 |
Pathak, Soham (9)
|
PVPM |
|
56.36 |
- |
|
Majeed-Hall, Jasper J (9)
|
PVCSC |
53.08 |
NS |
- |
|
Lim, Dylan S (10)
|
PVPM |
|
NS |
- |