| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cohen, Emily Z (16)
|
MRRFAC |
25.45 |
27.11 |
1.66 |
| 2 |
Colaizzo, Natalie A (16)
|
MRHVD |
26.64 |
27.45 |
0.81 |
| 3 |
DiNardo, Phoebe M (15)
|
MRHVD |
27.99 |
28.72 |
0.73 |
| 4 |
Cordeiro, Alex I (16)
|
MRHVD |
28.18 |
28.98 |
0.80 |
| 5 |
O'Donnell, Mairead E (17)
|
MRHVD |
28.03 |
29.78 |
1.75 |
| 6 |
Sanford, Lauren E (16)
|
MRNDAC |
28.71 |
30.06 |
1.35 |
| 7 |
Alcantara, Antonella L (15)
|
MRRFAC |
28.90 |
30.14 |
1.24 |
| 8 |
Murray, Gabriella R (15)
|
MRHVD |
28.25 |
30.84 |
2.59 |
| 9 |
Chant, Lucy J (15)
|
MRHVD |
29.50 |
32.09 |
2.59 |
| 10 |
Hernandez, Lexie R (15)
|
MRMHH |
33.16 |
33.24 |
0.08 |
| 11 |
Relyea, Carlie A (17)
|
MRHVD |
30.48 |
34.43 |
3.95 |