| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Konopka, Katrina M (22)
|
NCUN |
21.79 |
23.45 |
1.66 |
| 2 |
Kilpatrick, Ava S (15)
|
NCWNCY |
25.23 |
25.91 |
0.68 |
| 3 |
Hause, Elizabeth N (16)
|
SCYSSC |
|
26.97 |
- |
| 4 |
Little, Grace L (15)
|
SCYSSC |
25.98 |
27.27 |
1.29 |
| 5 |
Stull, Charlotte G (15)
|
SCYSSC |
25.83 |
27.39 |
1.56 |
| 6 |
Penny, Ella M (15)
|
SCYSSC |
25.01 |
27.51 |
2.50 |
| 7 |
Mazurowski, Katelyn A (16)
|
SCYSSC |
26.40 |
27.83 |
1.43 |
| 8 |
Agnew, Lauren M (16)
|
SCYSSC |
28.14 |
27.87 |
-0.27 |
| 9 |
Caggiano, Sophie G (17)
|
SCYSSC |
27.22 |
28.94 |
1.72 |
| 10 |
Martin, Caroline E (15)
|
SCYSSC |
28.25 |
29.22 |
0.97 |
| 11 |
Mastrogiacomo, Myah E (15)
|
SCYSSC |
30.24 |
29.87 |
-0.37 |
| 12 |
Browning, Taylor M (16)
|
SCYSSC |
28.57 |
30.12 |
1.55 |
| 13 |
Holland, Riley J (15)
|
NCWNCY |
35.77 |
36.51 |
0.74 |