| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gregory, Elizabeth F (10)
|
VAWAC |
39.85 |
40.85 |
1.00 |
| 2 |
Fary, Brenna E (10)
|
VAECAT |
42.28 |
41.85 |
-0.43 |
| 3 |
Campbell, Amelia D (10)
|
VASEVA |
46.37 |
42.30 |
-4.07 |
| 4 |
Rodrigues, Gabriella A (10)
|
VASEVA |
51.17 |
47.32 |
-3.85 |
| 5 |
Stupar, Aliyah G (10)
|
VASEVA |
47.79 |
47.34 |
-0.45 |
| 6 |
Aquino, Meagan E (10)
|
VAECAT |
45.33 |
47.41 |
2.08 |
| 7 |
Wojcik, Melissa A (10)
|
VASEVA |
48.68 |
48.33 |
-0.35 |
| 8 |
Shaltayeva, Victoria (10)
|
VASEVA |
48.52 |
48.44 |
-0.08 |
| 9 |
McGowan, Elly G (10)
|
VASEVA |
47.15 |
49.49 |
2.34 |
| 10 |
Phillips, Chloe E (10)
|
VAWAC |
45.66 |
49.77 |
4.11 |
| 11 |
Townsend, Leah C (10)
|
VAWAC |
54.50 |
52.44 |
-2.06 |
| 12 |
Kohler, Olivia A (10)
|
VATAC |
51.17 |
53.20 |
2.03 |
| 13 |
Hoover Smith, Taelyn R (10)
|
VAWAC |
|
55.19 |
- |
| 14 |
Graves, Bethany H (10)
|
VASEVA |
|
55.77 |
- |