| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gregory, Elizabeth F (10)
|
VAWAC |
35.46 |
35.77 |
0.31 |
| 2 |
Rodrigues, Gabriella A (10)
|
VASEVA |
42.73 |
43.18 |
0.45 |
| 3 |
Wojcik, Melissa A (10)
|
VASEVA |
48.60 |
46.66 |
-1.94 |
| 4 |
Aquino, Meagan E (10)
|
VAECAT |
44.89 |
47.28 |
2.39 |
| 5 |
Kohler, Olivia A (10)
|
VATAC |
50.47 |
48.12 |
-2.35 |
| 6 |
Shaltayeva, Victoria (10)
|
VASEVA |
49.89 |
48.48 |
-1.41 |
| 7 |
Landon, Emma M (10)
|
VAECAT |
|
48.59 |
- |
| 8 |
Campbell, Amelia D (10)
|
VASEVA |
53.41 |
51.21 |
-2.20 |
| 9 |
McGowan, Elly G (10)
|
VASEVA |
48.61 |
53.14 |
4.53 |
| 10 |
Phillips, Chloe E (10)
|
VAWAC |
54.17 |
56.52 |
2.35 |
| 11 |
Banks, Hannah M (10)
|
VASEVA |
1:07.17 |
1:02.09 |
-5.08 |
| 12 |
Hoover Smith, Taelyn R (10)
|
VAWAC |
|
1:02.99 |
- |
|
Graves, Bethany H (10)
|
VASEVA |
|
DQ |
- |