| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Olson, Emma G (12)
|
SRCSC |
43.66 |
37.88 |
-5.78 |
| 2 |
Kerr, Reese M (12)
|
SRBY |
|
42.13 |
- |
| 3 |
Dawson, Charlotte G (12)
|
SRBY |
43.24 |
42.49 |
-0.75 |
| 4 |
Hunsicker, Ann Y (11)
|
SRCSC |
42.94 |
42.64 |
-0.30 |
| 5 |
Leblanc, Remi J (12)
|
SRBY |
|
45.15 |
- |
| 6 |
Maymin, Edele (10)
|
SRUN |
|
47.28 |
- |
| 7 |
McFarland, Rebekah S (12)
|
SRBSC |
49.58 |
50.30 |
0.72 |
| 8 |
Lunde, Kennedy M (11)
|
SRBSC |
53.30 |
51.68 |
-1.62 |
| 9 |
Stoddard, Penny L (9)
|
SRBSC |
|
53.87 |
- |
| 10 |
Nickel, Brooklynn A (9)
|
SRBSC |
57.25 |
54.18 |
-3.07 |
| 11 |
Fairchild, Malia A (10)
|
SRBY |
1:11.56 |
57.66 |
-13.90 |
| 12 |
Smith, Lauren J (9)
|
SRBY |
|
1:00.52 |
- |
| 13 |
North, Sophronia M (10)
|
SRUNAT |
|
1:01.12 |
- |
| 14 |
Dawson, Lily J (9)
|
SRBY |
|
1:06.45 |
- |
| 15 |
Prince, Fiona D (11)
|
SRBY |
1:06.38 |
1:06.97 |
0.59 |
| 16 |
Gatzemeier, Mckinley M (9)
|
SRBSC |
|
1:19.16 |
- |
|
Leslie, Campbell J (9)
|
SRBY |
|
DQ |
- |