| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pierce, Deacon (10)
|
AMMIST |
40.94 |
41.37 |
0.43 |
| 2 |
Sarbak, Owen D (9)
|
AMPWRS |
44.13 |
42.21 |
-1.92 |
| 3 |
Moran, Aiden T (10)
|
NICLSC |
44.48 |
44.18 |
-0.30 |
| 4 |
Harris, Wesley S (9)
|
NIVICT |
45.81 |
45.38 |
-0.43 |
| 5 |
Callaway, Jackman A (10)
|
AMMIST |
48.00 |
45.75 |
-2.25 |
| 6 |
Ruffolo, Trenton J (9)
|
NIWWSC |
48.70 |
46.24 |
-2.46 |
| 7 |
Neal, Ducain H (9)
|
NIWWSC |
47.32 |
46.44 |
-0.88 |
| 8 |
Crowther, Finn L (10)
|
NIVELO |
45.35 |
46.71 |
1.36 |
| 9 |
Ray, Landon M (9)
|
NIVICT |
44.06 |
46.87 |
2.81 |
| 10 |
Heynes, Preston W (10)
|
AMPWRS |
55.38 |
47.63 |
-7.75 |
| 11 |
Battaglia, Benjamin P (10)
|
NIVICT |
49.88 |
47.77 |
-2.11 |
| 12 |
Donlon, Joseph R (10)
|
NIWWSC |
47.96 |
48.08 |
0.12 |
| 13 |
Coen, Mitchell R (10)
|
NISTAR |
50.92 |
48.30 |
-2.62 |
| 14 |
Rogers, Joseph T (9)
|
NISTAR |
47.99 |
48.68 |
0.69 |
| 15 |
Reygers, Camden M (10)
|
NIWWSC |
49.20 |
48.87 |
-0.33 |
| 16 |
Reimann, Nathan S (10)
|
NISTAR |
50.64 |
50.88 |
0.24 |
| 17 |
Wang, Michael (10)
|
NIVELO |
53.22 |
50.96 |
-2.26 |
| 18 |
Reukauf, Nathan A (9)
|
NISTAR |
52.48 |
51.12 |
-1.36 |
| 19 |
Nienhaus, Matthew (10)
|
NISHAQ |
53.19 |
51.37 |
-1.82 |
| 20 |
Kneeland, Noah J (9)
|
NIVELO |
47.62 |
51.48 |
3.86 |
| 21 |
Shepler, Jack (10)
|
NIVELO |
50.13 |
53.12 |
2.99 |
| 22 |
Broeker, Max E (10)
|
NIWWSC |
|
53.16 |
- |
| 23 |
Zuckerberg, Eben M (10)
|
NITTSC |
56.81 |
55.83 |
-0.98 |
| 24 |
Seplaki, Mark C (10)
|
NIVELO |
1:01.58 |
56.56 |
-5.02 |
| 25 |
Steele, Thomas J (9)
|
NIWEBS |
56.26 |
56.61 |
0.35 |
| 26 |
Fattey, Stephen C (9)
|
NISTAR |
57.30 |
57.10 |
-0.20 |
| 27 |
Young, Thaddeus M (9)
|
NISTAR |
58.12 |
58.37 |
0.25 |
| 28 |
Johnson, Logan J (10)
|
NISTAR |
53.36 |
58.85 |
5.49 |
| 29 |
Russell-Webster, Gabriel T (10)
|
NIBAAC |
1:16.45 |
59.32 |
-17.13 |
| 30 |
Schrack, Jacob A (9)
|
NIWEBS |
1:03.69 |
1:03.15 |
-0.54 |
| 31 |
Jank, Nathan P (9)
|
NIVELO |
1:03.83 |
1:03.39 |
-0.44 |
| 32 |
Miller, Bobby T (9)
|
NIVELO |
1:03.23 |
1:04.74 |
1.51 |
| 33 |
Kobielski, Carter E (10)
|
NIVELO |
1:04.90 |
1:06.12 |
1.22 |
| 34 |
Xu, Jeremy B (10)
|
NIVELO |
1:09.42 |
1:06.80 |
-2.62 |
| 35 |
Chandler, Oliver H (10)
|
NISTAR |
1:02.19 |
1:09.38 |
7.19 |
| 36 |
Reilly, Liam B (9)
|
NISTAR |
1:15.15 |
1:13.95 |
-1.20 |
|
Bard, Alex N (9)
|
AMPWRS |
|
DQ |
- |
|
Stancliff, John M (10)
|
NITTSC |
|
DQ |
- |
|
Bauman, Jackson T (9)
|
NISHAQ |
1:10.72 |
DQ |
- |
|
Basile, Ben A (9)
|
AMPWRS |
|
DQ |
- |