| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nhan, Madeleine S (10)
|
GUSSS |
3:01.37 |
2:53.75 |
-7.62 |
| 2 |
Voelker, Erin A (10)
|
GUAQUA |
3:12.68 |
2:58.25 |
-14.43 |
| 3 |
Anderson, Laine M (10)
|
GUAQUA |
3:36.40 |
3:06.66 |
-29.74 |
| 4 |
Crudo, Sofia I (10)
|
GUSSS |
3:07.97 |
3:06.71 |
-1.26 |
| 5 |
Klun, Marissa C (10)
|
GUAQUA |
3:19.67 |
3:11.74 |
-7.93 |
| 6 |
Pham, Kaitlyn C (9)
|
GUTEST |
|
3:18.36 |
- |
| 7 |
Hearon, Avery L (10)
|
GUAQUA |
3:42.44 |
3:19.55 |
-22.89 |
| 8 |
Ceraudo, Olivia M (10)
|
GUSHAC |
|
3:20.72 |
- |
| 9 |
Rayburn, Myah A (10)
|
GUAQUA |
3:23.75 |
3:20.98 |
-2.77 |
| 10 |
Londono, Caylee R (10)
|
GUSPA |
|
3:22.42 |
- |
| 11 |
del Valle - Guerra, Reese C (10)
|
GUAQUA |
3:45.45 |
3:26.42 |
-19.03 |
| 12 |
Welch, Taylor B (9)
|
GUAQUA |
3:29.86 |
3:27.28 |
-2.58 |
| 13 |
Laurito, Carmen S (9)
|
GUAQUA |
|
3:30.43 |
- |
| 14 |
Miller, Evie M (10)
|
GUSWAT |
|
3:31.48 |
- |
| 15 |
Tinson, Holly F (10)
|
GUAQUA |
|
3:34.58 |
- |
| 16 |
Luedde, Katie A (10)
|
GUSSS |
|
3:42.97 |
- |
| 17 |
Morgan, Jayde K (9)
|
GUSHAC |
4:05.24 |
3:44.49 |
-20.75 |
| 18 |
Warfield, McKayla L (10)
|
GUHCAP |
|
3:47.19 |
- |
|
Ferrero D'Ambrosio, Dana (10)
|
GUTTST |
3:20.00 |
DQ |
- |
|
Tonnesen, Molly L (10)
|
GUSPA |
|
NS |
- |
|
Sisung, Lilly L (9)
|
GUSHRK |
|
NS |
- |