| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
El Ghissassi, Awab (12)
|
NIVEST |
41.10 |
41.08 |
-0.02 |
| 2 |
Wettig, Gabriel J (12)
|
ADSCHE |
42.94 |
41.98 |
-0.96 |
| 3 |
Karaca, Rowan H (11)
|
ADSCHE |
43.75 |
42.31 |
-1.44 |
| 4 |
McIntyre, Derek P (12)
|
NEGMA |
43.27 |
42.81 |
-0.46 |
| 5 |
Weber, Stefan S (11)
|
CTRAC |
43.82 |
45.29 |
1.47 |
| 6 |
Leanza, Christopher M (12)
|
ADSCHE |
43.51 |
45.87 |
2.36 |
| 7 |
Hinds, Benjamin W (11)
|
NIWEBS |
50.56 |
46.05 |
-4.51 |
| 8 |
Splendido, Luke J (12)
|
ADSCHE |
45.68 |
46.23 |
0.55 |
| 9 |
Wakelee, Adam R (11)
|
NIVICT |
46.01 |
50.28 |
4.27 |
| 10 |
Bewlay, Charles E (9)
|
ADSCHE |
52.12 |
51.26 |
-0.86 |
| 11 |
Boenau, Tyler F (10)
|
ADBFIN |
57.88 |
56.37 |
-1.51 |
| 12 |
Liu, Vincent H (10)
|
ADSCHE |
1:03.66 |
57.25 |
-6.41 |
| 13 |
Kropp, Connor T (9)
|
ADEELS |
59.20 |
57.39 |
-1.81 |
| 14 |
Lewis, Christopher J (11)
|
NIVICT |
49.17 |
59.90 |
10.73 |
| 15 |
Steele, Thomas J (10)
|
NIWEBS |
1:01.95 |
1:01.49 |
-0.46 |
| 16 |
Kasper, Noah K (11)
|
ADSCHE |
1:01.71 |
1:01.98 |
0.27 |
| 17 |
Muncy, Graham M (9)
|
CTRAC |
1:04.17 |
1:06.74 |
2.57 |
| 18 |
Sellnow, Lucas M (9)
|
ADEELS |
|
1:12.52 |
- |
| 19 |
Choi, Ian M (10)
|
CTRAC |
52.69 |
1:56.69 |
64.00 |
|
Schrack, Jacob A (10)
|
NIWEBS |
1:15.00 |
NS |
- |