| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Morgan E (11)
|
NIVICT |
4:59.83 |
5:07.46 |
7.63 |
| 2 |
Braun, Gretchen L (11)
|
ADNTS |
4:56.77 |
5:07.70 |
10.93 |
| 3 |
O'Donnell, Maura E (12)
|
ADEELS |
5:22.39 |
5:29.04 |
6.65 |
| 4 |
Natemeier, Amelia E (12)
|
NIVICT |
5:51.74 |
5:37.32 |
-14.42 |
| 5 |
Wright, Becca M (12)
|
ADEELS |
5:43.88 |
5:39.99 |
-3.89 |
| 6 |
Fraley, Kaiya R (12)
|
ADBFIN |
5:56.57 |
5:43.42 |
-13.15 |
| 7 |
Huang, Madison Z (12)
|
ADSCHE |
5:39.96 |
5:43.96 |
4.00 |
| 8 |
Muncy, Madeline J (11)
|
CTRAC |
5:45.06 |
5:45.91 |
0.85 |
| 9 |
Cafarelli, Brianna N (12)
|
NIVICT |
5:44.66 |
5:53.68 |
9.02 |
| 10 |
Tolan, Sydney T (12)
|
ADSCHE |
6:07.01 |
5:55.04 |
-11.97 |
| 11 |
McFarland, Madeline J (11)
|
ADBFIN |
6:15.46 |
5:58.96 |
-16.50 |
| 12 |
Gilbert, Natalie J (10)
|
NIVICT |
5:47.14 |
6:00.39 |
13.25 |
| 13 |
Kropp, Paige H (11)
|
ADEELS |
6:45.20 |
6:03.63 |
-41.57 |
| 14 |
Fraley, Ella (10)
|
ADBFIN |
6:26.20 |
6:16.20 |
-10.00 |
| 15 |
Price, Catherine E (10)
|
ADSCHE |
6:31.17 |
6:34.26 |
3.09 |