| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Franklin, Delaney M (14)
|
SCSC |
2:43.32 |
2:41.55 |
-1.77 |
| 2 |
Sanders, Sydney M (14)
|
NCNCS |
2:44.52 |
2:44.07 |
-0.45 |
| 3 |
Rautaneva, Julia O (14)
|
STSTX |
2:43.44 |
2:45.30 |
1.86 |
| 4 |
Riggs, Caroline V (14)
|
WVWV |
2:46.53 |
2:45.91 |
-0.62 |
| 5 |
Foxhoven, Kyla M (14)
|
STSTX |
2:48.06 |
2:46.27 |
-1.79 |
| 6 |
Ivanov, Katherine A (13)
|
NCNCS |
2:44.07 |
2:46.51 |
2.44 |
| 7 |
Shahboz, Gwen J (13)
|
FLFL |
2:45.02 |
2:46.86 |
1.84 |
| 8 |
Freeman, Gabriella I (14)
|
GAGA |
2:39.75 |
2:47.78 |
8.03 |
| 9 |
Loving, Kylie R (14)
|
FLFL |
2:50.23 |
2:50.17 |
-0.06 |
| 10 |
Fernandez, Michelle A (14)
|
FGFG1 |
2:46.10 |
2:50.82 |
4.72 |
| 11 |
Roberts, Sofia M (14)
|
SESES |
2:49.71 |
2:51.38 |
1.67 |
| 12 |
Aouad, Christina M (13)
|
KYKY |
2:47.61 |
2:52.40 |
4.79 |
| 13 |
Holloway, Annsley B (14)
|
SESES |
2:49.56 |
2:52.64 |
3.08 |
| 14 |
Kanary, Karina M (14)
|
STSTX |
2:42.19 |
2:54.71 |
12.52 |
| 15 |
Entrekin, Evelyn E (14)
|
GAGA |
2:52.05 |
2:55.30 |
3.25 |
| 16 |
Han, Claire J (14)
|
SESES |
2:58.37 |
2:55.74 |
-2.63 |
| 17 |
Patterson, Lauren E (13)
|
NCNCS |
2:54.62 |
2:58.54 |
3.92 |
| 18 |
Havens, Elliott E (13)
|
KYKY |
2:55.44 |
3:00.02 |
4.58 |
| 19 |
Maeker, Abigail R (13)
|
NTNTX |
2:55.96 |
3:00.88 |
4.92 |
| 20 |
Frece, Avery G (13)
|
SCSC |
3:00.30 |
3:02.31 |
2.01 |
| 21 |
Ridenour, Olivia G (13)
|
WVWV |
3:05.65 |
3:04.96 |
-0.69 |
| 22 |
McGrath, Erin E (13)
|
NTNTX |
2:47.70 |
3:06.61 |
18.91 |
| 23 |
Howard, Melaina K (14)
|
STSTX |
3:09.04 |
3:07.33 |
-1.71 |
| 24 |
Riggs, Irene V (14)
|
WVWV |
3:11.41 |
3:09.25 |
-2.16 |
| 25 |
Kuprion, Lauren M (13)
|
KYKY |
3:02.94 |
3:09.91 |
6.97 |
| 26 |
Albritton, Molly A (14)
|
MSMS |
3:13.75 |
3:13.37 |
-0.38 |
| 27 |
Herrmann, Ella (14)
|
WTWT |
3:14.11 |
3:20.15 |
6.04 |
| 28 |
Luna, Hannah E (14)
|
WTWT |
3:18.60 |
3:22.20 |
3.60 |