| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Greeley, David A (15)
|
NCTAC |
|
21.27 |
- |
| 2 |
Wahlers, Ryan D (17)
|
NCTAC |
|
22.12 |
- |
| 3 |
Phifer, Benjamin A (17)
|
GASCAT |
|
22.16 |
- |
| 4 |
Summers, Kelsey T (16)
|
GADAQ |
|
22.30 |
- |
| 5 |
McKee, Garrett S (18)
|
NCWA |
|
22.67 |
- |
| 6 |
Cattanach, Quinn J (16)
|
GADAQ |
|
23.21 |
- |
| 7 |
Steelhammer, Gracie B (17)
|
NCMAC |
|
24.31 |
- |
| 8 |
Lillie, Nanami K (15)
|
GASCAT |
|
24.44 |
- |
| 9 |
McFaddin, Avery (16)
|
GASCAT |
|
24.67 |
- |
| 10 |
Abrahamse, Nadine H (18)
|
GADAQ |
|
24.98 |
- |
| 11 |
Anton, Isabella A (15)
|
NCMAC |
|
25.09 |
- |
| 12 |
Ayers, Skyler E (17)
|
SCSMRT |
|
25.34 |
- |
| 13 |
Schroeder, Lindsay P (16)
|
GADAQ |
|
25.66 |
- |