| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shao, Emma X (8)
|
ADGUY |
40.16 |
40.89 |
0.73 |
| 2 |
Adams, Michelle E (7)
|
ADCPP |
46.65 |
44.95 |
-1.70 |
| 3 |
Snow, Adalynn D (7)
|
AMUN |
43.03 |
45.10 |
2.07 |
| 4 |
Zhang, Annabelle Y (8)
|
ADCPP |
45.76 |
46.40 |
0.64 |
| 5 |
Wultsch-Fuller, Sonja E (8)
|
ADNTS |
57.16 |
48.82 |
-8.34 |
| 6 |
Stewart Fraser, Thornton R (8)
|
ADALBS |
|
49.43 |
- |
| 7 |
Sancilio, Elizabeth G (8)
|
ADDELM |
|
52.98 |
- |
| 8 |
Braun, Julia L (8)
|
ADNTS |
58.54 |
53.41 |
-5.13 |
| 9 |
Relyea, Cadence G (8)
|
ADDELM |
1:08.91 |
53.58 |
-15.33 |
| 10 |
Ryan, Callie J (8)
|
ADUSAM |
57.66 |
54.77 |
-2.89 |
| 11 |
MacLeod, Addison M (8)
|
ADUSAM |
1:00.77 |
55.56 |
-5.21 |
| 12 |
Colman, Maya K (8)
|
ADUN |
56.57 |
57.87 |
1.30 |
| 13 |
Ryan, Chloe M (8)
|
ADUSAM |
57.01 |
1:00.04 |
3.03 |
|
Holden, Alexandra E (8)
|
ADCPP |
|
DQ |
- |
|
Sekelj, Ella S (7)
|
ADNTS |
1:10.00 |
DQ |
- |