| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hollender, Gareth B (8)
|
VANOVA |
37.72 |
35.17 |
-2.55 |
| 2 |
Hotem, Andrew D (8)
|
VANOVA |
41.26 |
38.64 |
-2.62 |
| 3 |
Lowry, Chance P (8)
|
VANOVA |
|
38.75 |
- |
| 4 |
Jones, Gibson N (8)
|
VANOVA |
44.78 |
40.60 |
-4.18 |
| 5 |
Pew, Landon C (8)
|
VAPSDN |
44.57 |
43.70 |
-0.87 |
| 6 |
Maddux, Philip D (7)
|
VAPSDN |
45.71 |
45.52 |
-0.19 |
| 7 |
Eley, Jacob R (8)
|
VANOVA |
48.32 |
46.90 |
-1.42 |
| 8 |
Marston-Jennings, Chase C (8)
|
VAPSDN |
|
51.37 |
- |
| 9 |
Mercado, Jose D (8)
|
VAPSDN |
|
52.16 |
- |
| 10 |
Burns, III, William A (7)
|
VAPSDN |
|
55.22 |
- |
| 11 |
Liang, Henry Y (8)
|
VAPSDN |
57.11 |
55.54 |
-1.57 |
| 12 |
Temple, Christopher L (8)
|
VANOVA |
|
58.37 |
- |
|
Waterworth, William T (6)
|
VANOVA |
|
NS |
- |
|
Croxton, Alden R (8)
|
VAPSDN |
58.44 |
NS |
- |
|
Meyer, Luke C (8)
|
VAPSDN |
|
NS |
- |