| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hines, Audra J (9)
|
VABASS |
50.51 |
53.16 |
2.65 |
| 2 |
Crane, Berkeley M (9)
|
VADC |
57.02 |
57.02 |
0.00 |
| 3 |
Martinez, Anna S (10)
|
VANOVA |
57.37 |
57.63 |
0.26 |
| 4 |
Harvey, Parker J (9)
|
VANOVA |
57.92 |
58.06 |
0.14 |
| 5 |
Robertson, Logan A (10)
|
VAWAC |
56.60 |
58.22 |
1.62 |
| 6 |
Burdsall, Caroline E (9)
|
VANOVA |
59.09 |
59.65 |
0.56 |
| 7 |
Buyalos, Aubrey J (10)
|
VANOVA |
1:01.87 |
1:01.65 |
-0.22 |
| 8 |
Williams, Caroline H (9)
|
VANOVA |
1:01.41 |
1:02.59 |
1.18 |
| 9 |
Smolka, Rebecca B (9)
|
VANOVA |
1:09.23 |
1:05.20 |
-4.03 |
| 10 |
Sarna, Saachi (10)
|
VANOVA |
|
1:05.59 |
- |
| 11 |
Mungo, Livee E (9)
|
VANOVA |
|
1:10.98 |
- |
| 12 |
Rodriguez, Yuliana I (10)
|
VANOVA |
1:24.84 |
1:12.81 |
-12.03 |
| 13 |
Cumber, Margaret A (9)
|
VAWAC |
|
1:13.33 |
- |
|
Hoover Smith, Taelyn R (10)
|
VAWAC |
|
DQ |
- |
|
Radcliffe, Madeline S (10)
|
VANOVA |
|
NS |
- |