| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stuck, Kelsey M (11)
|
NCWOW |
31.52 |
31.11 |
-0.41 |
| 2 |
Metzinger, Mya Z (11)
|
NCWOW |
34.09 |
35.16 |
1.07 |
| 3 |
Weigand, Kelsey C (11)
|
NCWOW |
34.74 |
36.03 |
1.29 |
| 4 |
Bollenbacher, Heidi F (10)
|
NCWOW |
36.87 |
36.55 |
-0.32 |
| 5 |
McNamara, Cienna R (12)
|
NCWOW |
36.40 |
37.23 |
0.83 |
| 6 |
Steele, Eliana C (12)
|
NCWOW |
37.41 |
37.88 |
0.47 |
| 7 |
Bartlett, Kyla E (11)
|
NCWOW |
38.49 |
38.84 |
0.35 |
| 8 |
Pennington, Avery D (10)
|
NCWOW |
40.73 |
41.06 |
0.33 |
| 9 |
Dillon, Rylee P (12)
|
NCWOW |
43.70 |
41.53 |
-2.17 |
| 10 |
Norris, Chloe L (12)
|
NCWOW |
42.41 |
42.59 |
0.18 |
| 11 |
Carroll, Mackenzie M (10)
|
NCWOW |
42.40 |
42.99 |
0.59 |
| 12 |
Keeler, Samantha B (11)
|
NCWOW |
47.99 |
43.26 |
-4.73 |
| 13 |
Zimmerman, Mary C (10)
|
NCWOW |
45.32 |
44.48 |
-0.84 |
| 14 |
Derry, Violet M (10)
|
NCWOW |
43.09 |
44.66 |
1.57 |
| 15 |
Strickland, Lyla M (9)
|
NCWOW |
43.41 |
45.52 |
2.11 |
| 16 |
Phillips, Sarah C (9)
|
NCWOW |
43.04 |
46.18 |
3.14 |
| 17 |
Sterner, Adalyn G (11)
|
NCWOW |
44.33 |
46.40 |
2.07 |
| 18 |
Kraus, Katherine E (9)
|
NCWOW |
52.25 |
51.58 |
-0.67 |
| 19 |
Baynard, Melina K (9)
|
NCWOW |
50.98 |
51.97 |
0.99 |
| 20 |
DeSelms, Stella R (8)
|
NCWOW |
|
57.02 |
- |
| 21 |
Ezell-Tapia, Tiegan D (10)
|
NCWOW |
|
59.98 |
- |
|
McLean, Claire R (7)
|
NCWOW |
1:02.01 |
DQ |
- |