| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tuttle, Julia (14)
|
NTRWY |
30.10 |
29.40 |
-0.70 |
| 2 |
Meharg, Jubilee (14)
|
BCCY |
32.70 |
33.46 |
0.76 |
| 3 |
Hackney, Abigail (13)
|
BCCY |
34.07 |
33.97 |
-0.10 |
| 4 |
Walden, Riley M (13)
|
LARRAC |
35.15 |
34.75 |
-0.40 |
| 5 |
Pearce, Hannah R (13)
|
LARRAC |
34.80 |
34.87 |
0.07 |
| 6 |
McDonald, Madison (13)
|
BCCY |
39.09 |
37.72 |
-1.37 |
| 7 |
Phillip, Karuna (14)
|
PCY |
37.10 |
38.59 |
1.49 |
| 8 |
Mitchell, Katherine R (13)
|
LARRAC |
|
38.77 |
- |
| 9 |
Williams, Gracie (13)
|
BCCY |
51.36 |
45.14 |
-6.22 |
|
Duggan, Kendall F (13)
|
LARRAC |
|
DQ |
- |
|
Foshee, Nadia E (13)
|
LARRAC |
46.63 |
NS |
- |