| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Turner, Ryan T (14)
|
AMRACE |
24.19 |
23.66 |
-0.53 |
| 2 |
Tudorascu, Andrei (14)
|
AMMLAC |
23.83 |
24.10 |
0.27 |
| 3 |
Filer, Aiden S (14)
|
AMPTSC |
24.57 |
24.40 |
-0.17 |
| 4 |
Le, Kenneth B (14)
|
AMMLAC |
25.89 |
25.10 |
-0.79 |
| 5 |
Shipley, Maxwell J (14)
|
AMPEAQ |
26.36 |
25.55 |
-0.81 |
| 6 |
Klepchick, Matthew P (14)
|
AMMLAC |
26.20 |
25.92 |
-0.28 |
| 7 |
McClellan, Sean G (14)
|
AMURXA |
32.54 |
26.09 |
-6.45 |
| 8 |
Jacobs-Alarcon, Nicolas A (14)
|
AMURXA |
30.83 |
26.64 |
-4.19 |
| 9 |
Petkov, Alexander M (14)
|
AMPEAQ |
27.46 |
27.15 |
-0.31 |
| 10 |
Friday, Adam J (14)
|
AMPEAQ |
28.18 |
27.62 |
-0.56 |
| 11 |
Bishop, Luke S (14)
|
AMPEAQ |
28.41 |
27.74 |
-0.67 |
| 12 |
Jones, Asher R (14)
|
AMURXA |
31.31 |
28.40 |
-2.91 |
| 13 |
Dong, Boshi N (14)
|
AMPEAQ |
28.49 |
29.28 |
0.79 |
| 14 |
Hartman, Daniel (14)
|
AMMLAC |
28.23 |
29.48 |
1.25 |
| 15 |
Kriz, George A (14)
|
AMPTSC |
31.16 |
30.42 |
-0.74 |
| 16 |
Dwulit, Luke B (14)
|
AMMLAC |
32.32 |
31.58 |
-0.74 |