| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fronczek, Paige H (7)
|
AMMLAC |
46.65 |
43.46 |
-3.19 |
| 2 |
Turner, Alyssa J (7)
|
AMRACE |
42.23 |
45.54 |
3.31 |
| 3 |
Roncone, Aria R (7)
|
AMSFAC |
45.38 |
45.58 |
0.20 |
| 4 |
Chandiran, Sai Anshika (7)
|
AMPEAQ |
57.59 |
55.30 |
-2.29 |
| 5 |
Sinderson, Jessica L (7)
|
AMPEAQ |
59.78 |
58.43 |
-1.35 |
| 6 |
Robinson, Gabrielle D (7)
|
AMSFAC |
1:05.17 |
1:00.69 |
-4.48 |
| 7 |
Bittner, Mia A (7)
|
AMRXA |
56.58 |
1:00.77 |
4.19 |
| 8 |
McKinney, Bailey R (7)
|
AMRACE |
1:05.17 |
1:04.23 |
-0.94 |
| 9 |
Hartman, Caroline R (7)
|
AMPEAQ |
1:05.31 |
1:08.81 |
3.50 |
| 10 |
Pipes, Kayley G (7)
|
AMWAAC |
1:06.07 |
1:09.74 |
3.67 |
| 11 |
Mandel, Ryann C (7)
|
AMRXA |
1:30.99 |
1:13.77 |
-17.22 |
|
Ward, Ariana R (7)
|
AMWAAC |
1:03.85 |
NS |
- |