| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bauer, Mackenzie G (10)
|
AMPTSC |
44.32 |
43.40 |
-0.92 |
| 2 |
Bowers, Taylor J (10)
|
AMPTSC |
47.43 |
45.72 |
-1.71 |
| 3 |
Barnes, Cloey U (10)
|
AMSFAC |
49.87 |
50.34 |
0.47 |
| 4 |
Brennan, Elizabeth M (9)
|
AMSFAC |
51.28 |
51.78 |
0.50 |
| 5 |
Wilson, Lana M (9)
|
AMRXA |
1:00.70 |
52.25 |
-8.45 |
| 6 |
Stewart, Libby M (10)
|
AMRACE |
1:00.08 |
54.54 |
-5.54 |
| 7 |
Bandlamudi, Tanvisree S (8)
|
AMSFAC |
1:03.15 |
55.73 |
-7.42 |
| 8 |
Ziegler, Sasha E (9)
|
AMRXA |
56.93 |
55.81 |
-1.12 |
| 9 |
Valdia, Aanya (9)
|
AMSFAC |
1:12.19 |
57.21 |
-14.98 |
| 10 |
Ziegler, Ella E (9)
|
AMRXA |
57.60 |
58.35 |
0.75 |
| 11 |
Koot, Aubrey N (10)
|
AMPTSC |
1:06.00 |
1:02.12 |
-3.88 |
| 12 |
Robinson, Gabrielle D (7)
|
AMSFAC |
1:16.19 |
1:16.90 |
0.71 |
| 13 |
SenthilKumar, Mithra (8)
|
AMSFAC |
1:26.87 |
1:32.56 |
5.69 |
|
Casanova, Elena A (10)
|
AMBPR |
1:10.13 |
DQ |
- |
|
Bittner, Mia A (7)
|
AMRXA |
1:25.99 |
DQ |
- |