| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaguzis, Peri S (15)
|
AMKW |
26.74 |
26.89 |
0.15 |
| 2 |
Patterson, Delaney M (16)
|
AMBVSC |
26.29 |
26.93 |
0.64 |
| 3 |
Pohodich, Alexandra M (15)
|
AMBVSC |
26.34 |
27.49 |
1.15 |
| 4 |
Wagner, Halle E (17)
|
AMUN-M |
26.30 |
27.59 |
1.29 |
| 5 |
Fontanazza, Chloe G (15)
|
AMUNHA |
24.51 |
27.97 |
3.46 |
| 6 |
Glass, Rebecca P (15)
|
AMUN-M |
27.18 |
28.08 |
0.90 |
| 7 |
Jackovic, Emily A (16)
|
AMSPAC |
27.27 |
28.11 |
0.84 |
| 8 |
Stewart, Brooke M (17)
|
AMBVSC |
27.30 |
28.44 |
1.14 |
| 9 |
Fontanazza, Callysta L (17)
|
AMUNHA |
27.77 |
28.75 |
0.98 |
| 10 |
Malacki, Marina E (15)
|
AMSPAC |
27.32 |
28.90 |
1.58 |
| 11 |
Evans, Danika A (17)
|
AMSPAC |
28.09 |
29.63 |
1.54 |
| 12 |
Healey, Rebecca J (16)
|
AMBWA |
28.07 |
29.89 |
1.82 |