| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Noll, Natalie J (12)
|
AMBVSC |
2:29.72 |
2:23.01 |
-6.71 |
| 2 |
Catlin, Eliza C (12)
|
AMUN-M |
2:43.28 |
2:38.67 |
-4.61 |
| 3 |
Beam, Megan A (12)
|
AMSPAC |
2:51.34 |
2:41.96 |
-9.38 |
| 4 |
Klingensmith, Hanna (12)
|
AMNAC |
2:47.49 |
2:45.36 |
-2.13 |
| 5 |
Tornatore, Victoria G (12)
|
AMPTSC |
2:41.89 |
2:48.61 |
6.72 |
| 6 |
Hartman, Summer R (12)
|
AMUN-M |
2:45.86 |
2:50.07 |
4.21 |
| 7 |
Langlois, Mackenzie M (12)
|
AMPTSC |
2:43.20 |
2:51.15 |
7.95 |
| 8 |
Liu, Stacy R (11)
|
AMPTSC |
2:46.89 |
2:51.38 |
4.49 |
| 9 |
Augustynowicz, Macie A (11)
|
AMPTSC |
3:02.87 |
2:58.93 |
-3.94 |
| 10 |
Olson, Catherine (12)
|
AMPTSC |
3:08.47 |
2:59.21 |
-9.26 |
| 11 |
Carrozza, Gianna M (11)
|
AMKW |
3:30.02 |
3:28.95 |
-1.07 |
| 12 |
Collins, Hannah L (12)
|
AMUN-M |
4:11.10 |
3:42.64 |
-28.46 |