| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaguzis, Peri S (15)
|
AMKW |
55.77 |
58.10 |
2.33 |
| 2 |
McClain, Kaelyn R (17)
|
AMUNNA |
55.36 |
58.39 |
3.03 |
| 3 |
Wagner, Halle E (17)
|
AMUN-M |
57.40 |
59.10 |
1.70 |
| 4 |
Kwiatkowski, Megan R (15)
|
AMPTSC |
56.78 |
1:00.90 |
4.12 |
| 5 |
Madey, Kaylee A (17)
|
AMRESY |
57.47 |
1:01.30 |
3.83 |
| 6 |
Jackovic, Emily A (16)
|
AMSPAC |
1:01.18 |
1:01.38 |
0.20 |
| 7 |
Stewart, Brooke M (17)
|
AMBVSC |
58.47 |
1:01.70 |
3.23 |
| 8 |
Malacki, Marina E (15)
|
AMSPAC |
59.31 |
1:03.21 |
3.90 |
| 9 |
Pohodich, Alexandra M (15)
|
AMBVSC |
59.78 |
1:04.13 |
4.35 |
| 10 |
Morgan, Audra L (16)
|
AMBVSC |
1:08.61 |
1:04.16 |
-4.45 |
| 11 |
Majors, Allison E (15)
|
AMRESY |
1:03.93 |
1:04.19 |
0.26 |
| 12 |
Evans, Danika A (17)
|
AMSPAC |
1:03.66 |
1:07.21 |
3.55 |
| 13 |
Elder, Katrina R (15)
|
AMHAAC |
1:09.19 |
1:12.62 |
3.43 |
|
Rygelski, Grace H (15)
|
AMKW |
1:10.89 |
NS |
- |
|
Della Toffalo, Cara J (15)
|
AMRESY |
56.76 |
NS |
- |