| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Maddox H (8)
|
GUSSAN |
39.97 |
38.80 |
-1.17 |
| 2 |
Tautenhahn, Nicholas W (8)
|
GUSSAN |
41.11 |
38.87 |
-2.24 |
| 3 |
Medina, Felipe O (8)
|
GUCFSC |
40.53 |
39.72 |
-0.81 |
| 4 |
Ortez, Javier A (8)
|
GUCFSC |
41.40 |
41.99 |
0.59 |
| 5 |
Petzold, Hannes C (8)
|
GUTWST |
43.97 |
44.59 |
0.62 |
| 6 |
Connelly, Greyson L (8)
|
GUCFSC |
43.33 |
45.22 |
1.89 |
| 7 |
Corrick, Thomas M (8)
|
GUPACK |
47.62 |
45.57 |
-2.05 |
| 8 |
Thielen, Samuel A (8)
|
GUCFSC |
47.56 |
46.30 |
-1.26 |
| 9 |
Bykov, Matthew S (8)
|
GUDADS |
54.52 |
48.10 |
-6.42 |
| 10 |
Broderick, Richard L (8)
|
GUPACK |
51.24 |
48.31 |
-2.93 |
| 11 |
von Dohlen, Brayden M (8)
|
GUCFSC |
46.54 |
50.84 |
4.30 |
| 12 |
Marland, Nicholas E (8)
|
GUPACK |
52.22 |
51.97 |
-0.25 |
| 13 |
Vladimirov, Maksim (8)
|
GUCFSC |
56.95 |
56.20 |
-0.75 |
| 14 |
Wildermuth, Jack R (8)
|
GUPACK |
53.63 |
57.79 |
4.16 |
| 15 |
Walker, Gage M (8)
|
GUSSAN |
1:00.84 |
1:00.76 |
-0.08 |
| 16 |
Grubbs, William R (8)
|
GUPACK |
|
1:13.70 |
- |
| 17 |
Adams, Jasper Z (8)
|
GUAMBU |
1:16.93 |
1:24.32 |
7.39 |
|
Gallien, Connor J (8)
|
GUMAC |
52.16 |
NS |
- |
|
Abeid, Jonathan J (8)
|
GUTWST |
|
NS |
- |